Fig. 6

Attenuation of fluorescent signal by blood. a: Relative fluorescent signal in the injured knee, ΔFMT, depicted as function of joint diameter increase, Δdiameter, 24 h after induced joint bleed shows low ΔFMT at high Δdiameter. b: Excitation, fluorescence, and reconstructed image of a haemophilic mouse with a Δdiameter of 1.64 mm (a) and 0.49 mm (b) at 24 h. Colour bar shows intensity of signal. Note the attenuated signal in mouse a. c: Fluorescent signals measured with FMT after adding 500 pmol AngioSense750 to a 1.5 mL Eppendorf tube containing either 100% PBS; 50% v/v PBS and whole blood; or 100% whole blood (mean +/− standard deviation), as shown in (d)