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Table 1 Information of donors and intervertebral discs used in this study

From: Expression of the RANK/RANKL/OPG system in the human intervertebral disc: implication for the pathogenesis of intervertebral disc degeneration

Patient #

Age

Gender

Number of IVDs

MRI Grade

(disc level)

Experimental Protocol #

1

35–39

Male

2

2 (L1/2, L2/3)

1, 2, 5

2

35–39

Male

2

2 (T12/L1, L1/2)

1, 2

3

65–69

Male

2

4 (L3/4, L4/5)

1, 2

4

75–79

Female

1

5 (L3/4)

1

5

65–69

Male

2

4 (L3/4, L4/5)

1

6

25–29

Male

2

2 (L4/5, L5/S1)

2

7

65–69

Female

4

4 (L2/3, L4/5), 5 (L3/4), 3 (L5/S1)

2

8

80–84

Female

1

4 (L4/5)

3

9

70–74

Female

4

4 (L1/2-L4/5)

3

10

75–79

Female

3

4 (L2/3-L4/5)

3

11

65–69

Male

1

4 (L4/5)

3

12

75–79

Female

2

3 (L1/2), 4 (L4/5)

3

13

80–84

Female

2

4 (L3/4, L4/5)

4

14

70–74

Female

2

4 (T12/L1, L1/2)

4

15

50–54

Male

1

4 (L4/5)

4

16

65–69

Female

4

4 (L2/3-L5/S1)

4

17

75–79

Female

2

4 (L4/5, L5/S1)

4

18

70–74

Female

1

4 (L4/5)

4

19

75–79

Female

2

4 (L3/4, L4/5)

1, 4

20

40–44

Male

1

2 (L4/5)

4

21

55–59

Male

3

3 (L2/3, L3/4), 4 (L4/5)

4

22

65–69

Female

2

3 (L3/4), 4 (L4/5)

4

23

65–69

Male

1

4 (L5/S1)

4

24

80–84

Female

2

4 (L3/4, L4/5)

4

  1. Experimental protocols: 1. Effect of IL-1β stimulation on mRNA levels of RANK/RANKL/OPG; 2. Effect of RANKL on the expression of proinflammatory cytokines and matrix-degrading enzymes with or without IL-1β stimulation; 3. Effect of OPG on the expression of proinflammatory cytokines and matrix-degrading enzymes with or without IL-1β stimulation; 4. Effect of anti-human RANKL antibody on the expression of proinflammatory cytokines and matrix-degrading enzymes with or without IL-1β stimulation; and 5. Immunofluorescent analysis of human IVD cells