Fig. 2

Pain avoidance behavior tests. (a) Incapacitance test. Weight bearing asymmetry between ipsilateral (MIA side) and contralateral (control side) limbs was measured and the percent weight of ipsilateral limb was calculated as described previously [13]. The number of samples (rats) in each time point was as follows; High-dose (1.0 mg) group, days 0, 1, 3, 5, and 7: n = 12, day 14: n = 6. Low-dose (0.2 mg) group, days 0, 1, 3, 5, and 7: n = 8, day 14: n = 6. Steel tests were performed to calculate if the values in each time point were statistically different from those in day 0. Open circles; 0.2 mg group. Closed circles; 1.0 mg group. Asterisks indicate the p value is less than 0.05. (b) von Frey Hair test. Time course changes of paw withdrawal threshold in high-dose group. Steel tests were performed to calculate if the values in each time point were statistically different from that in day 0. Open triangles; Contralateral side. Closed triangles; Ipsilateral side. Asterisks indicate the p value is less than 0.05 (n = 12)