Table 2 ELISA Kits
From: The effect of autologous platelet rich plasma on tenocytes of the human rotator cuff
Analyte | ELISA Kit | Specifics | Dilution of PRP |
|---|---|---|---|
PDGF-AB | Quantikine human PDGF-AB (R&D systems) | Â | 1:50 |
IGF-I | Quantikine human IGF-I (R&D systems) | PRP needs pre-treatment (solution provided in the kit) | 1:100 (including pre-treatment) |
TGF-β1 | Quantikine human TGF-β1 (R&D systems) | PRP needs activation with 1 N HCL, neutralization with 1.2 N NaOH/0.5 M HEPES) | 1:40 (including activation) |
BMP-7 | DuoSet human BMP-7 (R&D systems) | Â | Undiluted |
BMP-12 (GDF-7) | Human GDF-7 ELISA Kit (Blue Gene) | Competitive ELISA | 1:2 |