Fig. 5
The expression of BMPR-IA gene in the transfected C3H10T1/2 cells was detected by Western blotting. a Western blot analysis of BMPR-IA protein levels in transfected C3H10T1/2 cells. Lane 1: C3H10T1/2 cells; Lane 2: C3H10T1/2/pcDNA3.1 cells; Lane 3: C3H10T1/2/pcDNA3.1/BMPR-IA (WT) cell; Lane 4 C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T) cells; Lane 5 C3H10T1/2/pcDNA3.1/BMPR-IA (MT 4A > C) cells; Lane 6 C3H10T1/2/pcDNA3.1/BMPR-IA (MT -349C > T and 4A > C) cells. b Statistical analysis of BMPR-IA protein expression levels in transfected C3H10T1/2 cells using the linear density ratio of BMPR-IA/GAPDH. The protein expression levels of BMPR-IA gene were significantly increased in C3H10T1/2/pcDNA3.1/BMPRIA (MT -349C > T, MT 4A > C, MT -349C > T and 4A > C) cells compared to the C3H10T1/2/pcDNA3.1/BMPRIA (WT) cells, C3H10T1/2/pcDNA3.1 cells, and C3H10T1/2 cells. n = 5, *p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA WT cells compared with C3H10T1/2 cells, *p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA WT cells compared with C3H10T1/2/pcDNA3.1 cells; **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T cells compared with C3H10T1/2 cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T cells compared with C3H10T1/2/pcDNA3.1 cells; **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT 4A > C cells compared with C3H10T1/2 cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT 4A > C cells compared with C3H10T1/2/pcDNA3.1 cells; **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T and 4A > C cells compared with C3H10T1/2 cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T and 4A > C cells compared with C3H10T1/2/pcDNA3.1 cells; **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T cells compared with C3H10T1/2/pcDNA3.1/BMPRIA WT cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT 4A > C cells compared with C3H10T1/2/pcDNA3.1/BMPRIA WT cells, **p < 0.001 C3H10T1/2/pcDNA3.1/BMPRIA MT -349C > T and 4A > C cells compared with C3H10T1/2/pcDNA3.1/BMPRIA WT cells