Fig. 6

In vivo gene expression of Pax7, Myf5, Myod1, Myog, Mstn, Myf6, Hgf, Met, Mef2c, Tgfb1 and protein expression of PAX7 and MYOG. a Pax7, Myf5 were significantly increased on day 2 in the Li-SWT group, while Met was significantly increased on day 3 in the Li-SWT group. No difference was found between treatment groups in the expression of Myod1, Myog, Myf6, Mstn, Hgf, Mef2c and Tgfb1. b Immunohistochemical staining for PAX7 was made for Li-SWT and controls and the expression on day 3 is shown. c The PAX7 stainings were quantified, and the highest number of satellite cells (PAX7+) was found as expected in regenerating areas of the muscle, however no difference was observed in the number of PAX7+ cells in Li-SWT compared to control. d The number of MYOG+ cells peaked on day 3 but no difference was observed between the Li-SWT and control group. MYOG stainings are shown for day 3. n = 3 in gene expressions and n = 4 in quantifications, * p < 0.05