Fig. 4
Quantifications of adherence of explants, outgrowth visualisations, cell adhesion and phalloidin staining of cells after shockwave treatment. a In explant cultures treated with 300 impulses of 0.1 mJ/mm2 46% of the treated explants adhered compared to 92% in controls (n = 48), however (b) the number of cells grown out from the explants and their expression of NCAM did not differ. c The experiment was then performed with human myoblasts isolated by enzyme dissociation. The cells were cultured on coverslips and harvested every 30 min the first 2 h; no difference in adherence was observed. (d) However, when the harvested coverslips were stained for F-actin with phalloidin, the shockwave treated cells displayed a much lower expression of F-actin, indicating a delayed actin assembly. The shown images are from cells harvested 90 min after culture.n = 3, * p < 0.05