Fig. 10

a Double staining (TNFR2/CD31) in section from specimen of 6 week experimental group; contralateral side. In the top, a blood vessel is seen being located outside a muscle fiber, showing CD31 IR (red colour) in the internal (intima) part and TNFR2 IR (green colour) in the outer part (large arrowhead). Arrows at nuclei located in peripheral parts of muscle fibers (marked with DAPI) in association with which TNFR2 IR is seen (appearing as fine dot-like whitish reactions). Fine CD31 immunoreactions (red colour) (small arrowheads), representing capillaries, and in which there are no TNFR2 immunoreactions, are also seen. There is a high auto fluorescence background reaction in the cytoplasm of the muscle fiber. Orig. magnif. ×300. b-d Figure showing double staining (IHC) for TNFR2 and the satellite cell marker Pax7. It is also labelled with DAPI. TNFR2 is not expressed in the satellite cells (arrows); there is no co-localization (b and c) (arrows at Pax7 positive cells). TNFR2 IR is on the other hand seen in some muscle cell nuclei (arrowheads, b and d). Sample from experimental animal; contralateral side (6 week group). Orig. magnif. ×300