Fig. 6
From: Co-expression of 1α-hydroxylase and vitamin D receptor in human articular chondrocytes
Alcian blue staining of spheroids maintained in control medium (a) 25(OH)D3 (b) or 1α,25(OH)2D3 at 10−7 M (c) for 2 weeks. Treated and untreated spheroids from three different donors histologically evaluated by Bern Score (d). Proliferation (e) of monolayer chondrocytes under continuous stimulation with 25(OH)D3, 1α,25(OH)2D3 or vehicle for 80 h in three donors. Ribbons represent 95% confidence interval and N indicates the time of normalization. Cartilage signature gene expression in monolayer cultures from three donors (f). Error bars represent one standard deviation, while horizontal bars and values represent p-values resulting from the comparison of means of the treated samples to the mean of the vehicle samples