Table 1 Primers used for real-time PCR experiments
Gene | Primer sequence | |
|---|---|---|
PAI-1 | Forward | 5′-TTCAGCCCTTGCTTGCCTC-3’ |
Reverse | 5′-ACACTTTTACTCCGAAGTCGGT-3’ | |
TGF-β1 | Forward | 5′-CCTCTGTCACCTGCTCAACA-3’ |
Reverse | 5′-GATGAATTGGCGTGGAATCT-3’ | |
Runx2 | Forward | 5′-AAATGCCTCCGCTGTTATGAA-3’ |
Reverse | 5′-GCTCCGGCCCACAAATCT-3’ | |
Osterix | Forward | 5′-AGCGACCACTTGAGCAAACAT-3’ |
Reverse | 5′-GCGGCTGATTGGCTTCTTCT-3’ | |
ALP | Forward | 5′-ATCTTTGGTCTGGCTCCCATG-3’ |
Reverse | 5′-TTTCCCGTTCACCGTCCAC-3’ | |
OCN | Forward | 5′-CCTGAGTCTGACAAAGCCTTCA-3’ |
Reverse | 5′-GCCGGAGTCTGTTCACTACCTT-3’ | |
RANKL | Forward | 5′- CACAGCGCTTCTCAGGAGCT-3’ |
Reverse | 5′-CATCCAACCATGAGCCTTCC-3’ | |
OPG | Forward | 5′-AGTCCGTGAAGCAGGAGT-3’ |
Reverse | 5′-CCATCTGGACATTTTTTGCAAA-3’ | |
GAPDH | Forward | 5′-AGGTCGGTGTGAACGGATTTG-3’ |
Reverse | 5′-GGGGTCGTTGATGGCAACA-3’ |