Fig. 3
Effects of IL-1β on the expressions of PAI-1 and TGF-β1 in primary mouse osteoblasts. a Primary osteoblasts were obtained from 3-day-old female WT mice. Total RNA was extracted from primary osteoblasts cultured with or without 10 ng/mL IL-1β or 1 ng/ml TGF-β1 for 24 h. The levels of PAI-1 or GAPDH mRNA were assessed by real-time PCR. Data represent the mean ± SEM. n = 6 for each group. **p < 0.01. b Primary osteoblasts were obtained from 3-day-old female WT and PAI-1 KO mice. Total RNA was extracted from primary osteoblasts cultured with or without 10 ng/mL IL-1β for 24 h. Then, the levels of TGF-β1 or GAPDH mRNA were assessed by real-time PCR. Data represent the mean ± SEM. n = 6 for each group