Protein | Fold increase |
p
|
|---|
Control | TNF-α | TNF-α + 5Z | Control vs TNF-α | TNF-α vs TNF-α +5Z | Control vs TNF-α +5Z |
|---|
ADAMTS-4 | 1.0 ± 0.2 | 21.5 ± 2.8a
| 1.4 ± 0.3b
| 0.015 | 0.015 | 0.103 |
MMP-3 | 1.0 ± 0.2 | 2.0 ± 0.1a
| 0.5 ± 0.1a,b
| 0.001 | 1 × 10−6
| 0.015 |
COX-2 | 1.0 ± 0.3 | 3.0 ± 0.1a
| 2.2 ± 0.1a
| 0.001 | 0.052 | 0.022 |
mPGES1 | 1.0 ± 0.1 | 2.7 ± 0.1a
| 1.5 ± 0.1a,b
| 0.004 | 0.006 | 0.027 |
NGF | 1.0 ± 0.2 | 2.2 ± 0.6a
| 0.5 ± 0.1a,b
| 0.032 | 0.017 | 0.009 |
- Protein analysis for matrix metalloproteinase-3 (MMP-3), a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 4 (ADAMTS-4), cycloxygenase-2 (COX-2), microsomal prostaglandin E synthase-1, and nerve growth factor (NGF) gene expression in synovial cell culture. Synovial cells were stimulated with human recombinant 10 ng/mL TNF-α (TNF-α), or 10 ng/ml TNF-α and 10 μM (5Z)-7-oxozeaenol (TNF-α + 5Z) for 24 h prior to the protein extraction. Expression of ADAMTS-4, COX-2, and mPGES1 proteins were analyzed by western blotting analysis. MMP3 and NGF protein levels in synovial cell culture supernatants were measured by ELISA. All data are presented as the mean ± standard error (n = 6). a
p < 0.05 compared with the untreated control. b
p < 0.05 compared with the TNF-α
