Fig. 1
From: Impairment of early fracture healing by skeletal muscle trauma is restored by FK506
Experimental time course. The schematic depicts the therapeutic regimen (FK506 or saline) and experimental assays carried out over the course of 28Â days post OSTÂ +Â VML. Trauma consisting of OSTÂ +Â VML was generated at day 0. FK506 or saline control was administered daily by the intraperitoneal route from day 0 to day 14 following injury. At 3Â days post-injury, flow cytometry and immunohistochemical analysis of damaged muscle and adjacent fracture region was carried out, immunohistochemical analysis was also analyzed at 14Â days post-injury. At 28Â days post-trauma strength testing assays of bone and muscle were carried out