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Table 2 PCR primers

From: Immunogenicity and immunomodulatory effects of the human chondrocytes, hChonJ

Gene name Sequence Amplicon size (bp)
HLA-ABC (MHC class I) Sense, 5′-GATTCTCCCCAGACGCCGAG-3′
Antisense, 5′-CCTGGGCACTGTCACTGCTT-3′
1082
CD274 (PD-L1) Sense, 5′-CTACCCCAAGGCCGAAGTCA-3′
Antisense, 5′-CCCAGAATTACCAAGTGAGTCC-3′
258
CD273 (PD-L2) Sense, 5′-ACCCTGGAATGCAACTTTGACACT-3′
Antisense, 5′-ACTTGGACTTGAGGTATGTGGAACGA-3′
167
CD80 Sense, 5′-CATCACGGAGGGTCTTCTAC-3′
Antisense, 5′-AGGATCTTGGGAAACTGTTGT-3′
710
CD86 Sense, 5′-TGCAAACTCTCAAAACCAAAG-3′
Antisense, 5′-AAAACACGCTGGGCTTCATCA-3′
815
IDO Sense, 5′-AGGCAACCCCCAGCTATCAGAC-3′
Antisense, 5′-TCAGGGAGACCAGAGCTTTCACAC-3′
314
β-actin Sense, 5′-GCTCGTCGTCGACAACGGCTC-3′
Antisense, 5′-CAAACATGATCTGGGTCATCTTCTC-3′
329