Table 2 PCR primers
From: Immunogenicity and immunomodulatory effects of the human chondrocytes, hChonJ
Gene name | Sequence | Amplicon size (bp) |
|---|---|---|
HLA-ABC (MHC class I) | Sense, 5′-GATTCTCCCCAGACGCCGAG-3′ Antisense, 5′-CCTGGGCACTGTCACTGCTT-3′ | 1082 |
CD274 (PD-L1) | Sense, 5′-CTACCCCAAGGCCGAAGTCA-3′ Antisense, 5′-CCCAGAATTACCAAGTGAGTCC-3′ | 258 |
CD273 (PD-L2) | Sense, 5′-ACCCTGGAATGCAACTTTGACACT-3′ Antisense, 5′-ACTTGGACTTGAGGTATGTGGAACGA-3′ | 167 |
CD80 | Sense, 5′-CATCACGGAGGGTCTTCTAC-3′ Antisense, 5′-AGGATCTTGGGAAACTGTTGT-3′ | 710 |
CD86 | Sense, 5′-TGCAAACTCTCAAAACCAAAG-3′ Antisense, 5′-AAAACACGCTGGGCTTCATCA-3′ | 815 |
IDO | Sense, 5′-AGGCAACCCCCAGCTATCAGAC-3′ Antisense, 5′-TCAGGGAGACCAGAGCTTTCACAC-3′ | 314 |
β-actin | Sense, 5′-GCTCGTCGTCGACAACGGCTC-3′ Antisense, 5′-CAAACATGATCTGGGTCATCTTCTC-3′ | 329 |