Fig. 2

Flow cytometry analysis for the change of immune-related surface markers on IFN-γ treated hChonJ. After hChonJ cells were exposed to 300 IU/mL of IFN-γ, the cells were labeled with antibodies against immune-related surface markers, including MHC class I (HLA-ABC), MHC class II (HLA-DR), co-stimulatory molecules (CD80 and CD86), co-inhibitory molecules (PD-L1 and PD-L2) and immunoglobulin G1 isotype control. The expression of MHC class I and II molecules (a) was increased, but there was no change in the expression level of the co-stimulatory molecules CD80 and CD86 (b). In the case of co-inhibitory molecules, PD-L1 expression was increased and the expression of PD-L2 was detected which was not expressed on IFN-γ untreated hChonJ cells (c). These results are the representative of at least 3 independent experiments