Fig. 1

Flow cytometry analysis for immune-related surface marker on hChonJ. hChonJ cells were labeled with antibodies against immune-related surface markers, including MHC class I (HLA-ABC), MHC class II (HLA-DR), co-stimulatory molecules (CD80 and CD86), co-inhibitory molecule, (CD274 (PD-L1) and CD273 (PD-L2)) and immunoglobulin G1 isotype control. Raji cells were used as controls. hChonJ cells express MHC class I (a), but not MHC class II (a) and co-stimulatory molecules CD80, CD86 (b). In addition, hChonJ express low level of PD-L2, but not PD-L1 (c). RT-PCR confirmed the expression of PD-L2 in hChonJ (d). These results are the representative of at least 3 independent experiments