Fig. 6

Immunohistochemical localization of antigens detected by anti-CCP antibodies in synovial tissues. Immunohistochemistry with anti-circulated citrullinated peptide (anti-CCP) antibody (Abbiotec), brown staining of the lining and sublining in the inflamed synovial tissue from wild-type collagen-induced arthritis (CIA) mice (f and g) and Padi4^−/− CIA mice (b and c) and in the synovial tissue from wild-type control mice (e) and Padi4^−/− control mice (a). Staining of a matched irrelevant rabbit IgG-negative control was performed at the same concentration (d and h). In OA (i) and RA synovial tissue samples (j), CCP antigens were observed in the synovial sublining and lining layer. Magnification is × 40, except in c, g and j lower (×100)