Fig. 5
From: Aggrecan heterogeneity in articular cartilage from patients with osteoarthritis
Agarose gel electrophoresis of aggrecan treated with different proteases. Proteoglycan from guanidine extracts of neonatal cartilage and OA articular cartilage (M5) were analyzed by electrophoresis on agarose gels following digestion with trypsin, MMP3 or ADAMTS5. Aggrecan was visualized by staining the gel with Toluidine blue. The OA cartilage samples were obtained midway between the lesion and the joint margin from the femoral condyle (M5). The migration position of isolated chondroitin sulfate chains (CS) is also presented to indicate the migration position of the small proteolytic fragments