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Table 2 Effect of IL-1β and TNF-α on secretion of different molecules by human chondrocytes

From: α-Melanocyte-stimulating-hormone (α-MSH) modulates human chondrocyte activation induced by proinflammatory cytokines

 

Stimulus

Molecules in the Supernatants (ng/mL)

Medium

IL-1β

TNF-α

IL-6

3.0 ± 0.2

367 ± 4.9*

70 ± 3.7*

IL-8

0.0

781 ± 32*

120 ± 5.6*

MMP-3

2.0 ± 0.00

14500 ± 400*

8100 ± 50*

MMP-13

8.1 ± 0.5

71.0 ± 8.0*

35.3 ± 2.7*

TIMP-3

1.63 ± 0.12

2.71 ± 0.05°

1.23 ± 0.01 ns

TIMP-4

0.11 ± 0.01

0.12 ± 0.01 ns

0.12 ± 0.01 ns

NO (μM)

19.5 ± 3.5

102.8 ± 32.6*

95.8 ± 36.9*

  1. Primary human chondrocytes were cultured for 40 h in medium or in the presence of 10 ng/mL IL-1ß or TNF-α. The cell-free supernatants were collected and the concentration of interleukins, MMPs, TIMPs and NO were measured. Data from 4 separate experiments (± S.E.M.) are shown
  2. * p < 0.0001; °p < 0.002; ns: not significant vs medium
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BMC Musculoskeletal Disorders

ISSN: 1471-2474

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