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Table 2 Effect of IL-1β and TNF-α on secretion of different molecules by human chondrocytes

From: α-Melanocyte-stimulating-hormone (α-MSH) modulates human chondrocyte activation induced by proinflammatory cytokines

  Stimulus
Molecules in the Supernatants (ng/mL) Medium IL-1β TNF-α
IL-6 3.0 ± 0.2 367 ± 4.9* 70 ± 3.7*
IL-8 0.0 781 ± 32* 120 ± 5.6*
MMP-3 2.0 ± 0.00 14500 ± 400* 8100 ± 50*
MMP-13 8.1 ± 0.5 71.0 ± 8.0* 35.3 ± 2.7*
TIMP-3 1.63 ± 0.12 2.71 ± 0.05° 1.23 ± 0.01 ns
TIMP-4 0.11 ± 0.01 0.12 ± 0.01 ns 0.12 ± 0.01 ns
NO (μM) 19.5 ± 3.5 102.8 ± 32.6* 95.8 ± 36.9*
  1. Primary human chondrocytes were cultured for 40 h in medium or in the presence of 10 ng/mL IL-1ß or TNF-α. The cell-free supernatants were collected and the concentration of interleukins, MMPs, TIMPs and NO were measured. Data from 4 separate experiments (± S.E.M.) are shown
  2. * p < 0.0001; °p < 0.002; ns: not significant vs medium
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