Fig. 3
From: Type II collagen and glycosaminoglycan expression induction in primary human chondrocyte by TGF-β1
The main function of hChonJb#7 as the source of active TGF-β1. a The hChonJ micro-masses were cultured with medium including rhTGF-β1 (25 ng/ μ-mass), purified human type II collagen (25 ng/ μ-mass) or proteoglycan human joint cartilage extract (25 ng/ μ-mass). After 1 week, total RNA was purified from each micro-mass and applied to qPCR to measure the type II collagen gene expression level. The Y-axis shows fold difference of type II collagen mRNA level increased inTGF-β1 supplemented micro-masses compared to hChonJ micro-masses. The error bars represent the standard deviation. b Micro-masses cultured in the same method to (a) used to measure the accumulated GAG amount. The error bars represent the standard deviation