Figure 5

The relative quantification (mean ± standard deviation) of sialoprotein (a) and runx-2 (b) transcripts as measured by real-time RT-PCR in calvarial osteoblasts taken from newborn control rats and those exposed to various concentrations of caffeine (25, 50, 100 mg/Kg) during pregnancy and subsequently cultured in osteogenic differentiation medium for 7, 14 or 21 days. *p <0.05, **p < 0.01, ***p < 0.001.