Sphingosine-1-phosphate attenuates proteoglycan aggrecan expression via production of prostaglandin E2 from human articular chondrocytes

Table 1 primer sequences

specificity	sense (5'-3) antisense (5'-3)	bp	Ref. [GenBank accession nos]
EDG1	TCC TTC ACT TTA GTT TCA AAC CCA AGT	160	[GenBank:NM-001400]
	CAA CCC CAG CTC TGA TAA CTC TCA
EDG2	ATT TTG TGT GGT TTG GTG CAA GTC	163	[GenBank:NM-057159]
	AGC AGT CCA TAG TCT CAT CCA TCA C
EDG3	AAA ATT AGC TGG ATG CGA TAG CAC	156	[GenBank:NM-005226]
	TTT GAG ACA GAG GGT CGC TCT GT
EDG4	CTG ACT GGC AGG ACA AGG TCT G	164	[GenBank:NM-004720]
	ACC CCC TAA ACC TGA GAT GGC T
EDG5	CCA AGC ATT ATG TGC TGT GC	206	[GenBank:NM-004230]
	CAG AAG GAG GAT GCT GAA GG
EDG6	CTA CTC CAA GCG CTA CAT CCT CT	172	[GenBank:NM-003775]
	GAT CAT CAG CAC CGT CTT CAG
EDG7	CTC ATC ATG GTT GTG GTG TAC CTG	156	[GenBank:NM-012152]
	GCA TAC CAC AAA CGC CCC TAA G
EDG8	AGG ACC TTG TGG GTG ATA TAG AGG AC	156	[GenBank:NM-030760]
	CCC CTT CAC CTT CTC TGG TTT TC
COX2	TTC TGA GAT TGT GGG AAA ATT GCT	305	[GenBank:M90100]
	AGA TCA TCT CTG GCT GAG TAT CTT
GAPDH	CCA CCC ATG GCA AAT TCC ATG GCA	598	[GenBank:NM-002046]
	TCT AGA CGG CAG GTC AGG TCC ACC		[24]

ISSN: 1471-2474