Figure 3
From: Dexamethasone stimulates expression of C-type Natriuretic Peptide in chondrocytes
DEX regulates expression of CNP and CNP receptors. Primary chondrocytes were incubated with DMSO or 10-7 M DEX for 12 to 72 hours, and Nppc (encoding CNP, A), Npr2 (encoding the CNP signaling receptor, B) and Npr3 (encoding the CNP decoy receptor, C) mRNA levels were determined by real-time PCR relative to Gapdh. Nppc expression is significantly increased upon DEX treatment, while DEX down-regulated Npr2 slightly and up-regulated Npr3 at the 48 and 72 hr time points. Data represent mean ± SEM from three or four independent trials (*P < 0.05).