Figure 2
Effect of 1,25D 3 alone on synthesis of RANK by cultured human macrophages. Macrophages were cultured at 5 × 105/well for 7 days. 1,25D3 (10-7M or 10-9M) was added. Macrophages were immunostained for RANK with a specific polyclonal antibody. Cells exposed to 10-7M 1,25D3 (a) synthesised more RANK than cells exposed to 10-9M 1,25D3 (b). Cultures supplemented with 1,25D3 synthesised more RANK than control cultures without 1,25D3 (c).