Table 1 Primer pairs and oligonucleotide probes used in this study.
From: Delayed union of femoral fractures in older rats:decreased gene expression
Gene | Amplimer | Annealing T | Sense | Antisense | Probe | Accession |
|---|---|---|---|---|---|---|
Size (bp) | Temp. (C) | |||||
Osteocalcin | 411 | 60 | TCTCTCTGCTCACTCTGCTG | ATTTTGGAGCAGCTGTGCCG | AGGTGGTGAATAGACTCCGG | M23637 |
Type I Collagen | 410 | 62 | AGGGACACAGAGGTTTCAGTG | ACCATTGGCACCTTTAGCACC | ATCGTTACCACGAGCACCAG | Z78279 |
Type II Collagen | 563 | 62 | TCATAGGACCTAAAGGACCTCC | TTCTCCCTTAGCACCATCGAG | AACTTTGCAGCCCAGATGGCT | L48440 |
BMP-2 | 570 | 60 | ACGTCCTCAGCGAGTTTGAG | CACCTGGCTTCTCCTCTAAG | CTTCCCGACGCTTCTTCTTC | Z25868 |
BMP-4 | 601 | 60 | ACTTCTGCAGATGTTTGGGCT | AGGTAACGATCGGCTAATCCT | CTATTTCGGGAGCAGGTGGA | Z22607 |
BMP-receptor | 472 | 58 | TTGTCGCCATGATCGTCTTC | AGGAAGTCATAGAGAGACCC | TAAAGGCCGGTATGGAGAAG | D38082 |
18S rRNA | 321 | 66 | AGGCCCTGTAATTGGAATGAGTC | TTATTCCTAGCTGCGGTATCCAG | AGCTCGTAGTTGGATCTTGG | K01593 |