Table 2 Primer sequences for RT-PCR analysis
From: Histone deacetylase 4 alters cartilage homeostasis in human osteoarthritis
Gene | Primer |
|---|---|
MMP1 | Forward: 5′-AAAATTACACGCCAGATTTGCC-3′ |
|  | Reverse: 5′-GGTGTGACATTACTCCAGAGTTG-3′ |
MMP3 | Forward: 5′-AGTCTTCCAATCCTACTGTTGCT-3′ |
|  | Reverse: 5′-TCCCCGTCACCTCCAATCC-3′ |
MMP13 | Forward: 5′-ACTGAGAGGCTCCGAGAAATG-3′ |
|  | Reverse: 5′-GAACCCCGCATCTTGGCTT-3′ |
ADAMTS4 | Forward: 5′-GAGGAGGAGATCGTGTTTCCA-3′ |
|  | Reverse: 5′-CCAGCTCTAGTAGCAGCGTC-3′ |
ADAMTS5 | Forward: 5′-GAACATCGACCAACTCTACTCCG-3′ |
|  | Reverse: 5′-CAATGCCCACCGAACCATCT-3′ |
Aggrecan | Forward: 5′-ACTCTGGGTTTTCGTGACTCT-3′ |
|  | Reverse: 5′-ACACTCAGCGAGTTGTCATGG-3′ |
COL2A1 | Forward: 5′-TGGACGATCAGGCGAAACC-3′ |
|  | Reverse: 5′-GCTGCGGATGCTCTCAATCT-3′ |
Beta-Actin | Forward: 5′-CATGTACGTTGCTATCCAGGC-3′ |
|  | Reverse: 5′-CTCCTTAATGTCACGCACGAT-3′ |