Effect of cycle number on mRNA expression and protein level of collagen type I and III. The high cycle number (1000) caused elevated Collagen type I mRNA levels (p < 0.05). Pro-collagen type III protein levels (C) were upregulated in the 100 cycles regimen (p < 0.05, 24 h). The *indicates a significant difference of p < 0.05, the indicates ƚ significant difference of p < 0.01, the error bars indicate the standard error. All data points from each group were normalized to unstretched controls harvested at every time point.