Table 3 RFLP enzymes description
From: Estrogen receptors genes polymorphisms and age at menarche in idiopathic scoliosis
SNP | Enzyme | Position | Recognition site 5’- > 3’ |  | Allele | Digestion product length |
|---|---|---|---|---|---|---|
rs9340799 | XbaI | ESR1 | T*CTAGA | T*CTA GA | A | 910Â bp + 390Â bp |
Intron 1 | TCTG GA | G | 1300Â bp | |||
351A > G | ||||||
rs2234693 | PvuII | ESR1 | CAG*CTG | CAG*CT G | T | 271Â bp + 488Â bp |
Intron 1 | CAGCC G | C | 759Â bp | |||
397C > T | ||||||
rs4986938 | AluI | ESR2 | AG*CT | A G*CT | A | 445 pz + 201 pz |
UTR | G GCT | G | 646 pz | |||
1730G > A | ||||||
rs1256049 | RasI | ESR2 | GT*AC | GT*A C | A | 293 pz + 289 pz |
Exon 5 | GTG C | G | 582 pz | |||
1082G > A |