Table 2 Primers’ description
From: Estrogen receptors genes polymorphisms and age at menarche in idiopathic scoliosis
Gene | SNP | Enzyme | 5’- > 3’ | Sequence 5’- > 3’ | Annealing temp | Amplicon length |
|---|---|---|---|---|---|---|
ESR1 | rs9340799 | XbaI | F | CTGCCACCCTATCTGTATCTTTTCCTATTCTCC | 71°C | 1300 bp |
| Â | Â | Â | R | TCTTTCTCTGCCACCCTGGCGTCGATTATCTGA | ||
|  | rs2234693 | PuvII | F | AGGCTGGGCTCAAACTACAG | 60°C | 759 bp |
| Â | Â | Â | R | TCCTTGGCAGATTCCATAGC | ||
ESR2 | rs1256049 | Rsal | F | TTCTGAGCCGAGGTCGTAGT | 66°C | 582 bp |
| Â | Â | Â | R | TGAATCCTTGGACCCAACTC | ||
|  | rs4986938 | AluI | F | GTGTGTGGTGGGACACAGAG | 65°C | 646 bp |
| Â | Â | Â | R | AGGCCATTGAGTGTGGAAAC |