Table 1 PCR primer sequences for COL9A1, COL9A2, COL9A3, COMP, and MATN3
From: Novel COL9A3 mutation in a family diagnosed with multiple epiphyseal dysplasia: a case report
Primer sequences | |||
|---|---|---|---|
COL9A1 gene | Tm (°C) | Product size (bp) | |
Exon 8-10 | F - CCGATGTGCTCCACTAACCT | 56 | 824 |
R - GGCCAAGTTTAGAGCCACAG | |||
COL9A2 gene | |||
Exon 2 | F - CAGCTTCCTGCACTGTCTGA | 56 | 241 |
R - GACGAGGGGCACTACATCTC | |||
Exon 3-4 | F - TGAGCCGTAGTGTGCTGTCT | 56 | 278 |
R - CTGGAGGTCAATTGGCAGAG | |||
COL9A3 gene | |||
Exon 2 | F - TTTGGGTCTCACCGAGGA | 56 | 293 |
R - GCCTGGTTTTCTCTCCATCA | |||
Exon 3 | F - CTTGAGGGACCCCTGATTTT | 56 | 152 |
R - TGTTCTGAGTTCCCCCTTTC | |||
Exon 4 | F - GCATTTTGCTTCATTGCTGA | 56 | 222 |
R - AATTAGGGCCGGACTCCTC | |||
COMP gene | |||
Exon 8-9 | F - TTGAGGCGGGGTTGGGTG | 64 | 413 |
R - ACCGTGCCGAGCCGTAGAT | |||
Exon 10 | F - AGGAGTGTGACCTTTGCCTTCT | 64 | 334 |
R - CTAGTCCAGCTTACCCCATCC | |||
Exon 11-12 | F - GAAGTCATTCTGGCCTGGTC | 64 | 518 |
R - AGCGTTTTGTCAAAGGCTACC | |||
Exon 13 | F - CGGGTAGCCTTTGACAAAACG | 62 | 331 |
R - GCCCGCCCACCGTAGAC | |||
Exon 14-15 | F - GGCGGGCCCTGACTTTAG | 64 | 546 |
R - ATAACCCCGCCCCTCTGT | |||
Exon 16 | F - GTTCTGGGTGCCAGGTTC | 64 | 335 |
R - AAGGGTTTTACGGAGGGTCAT | |||
Exon 17 | F - TGCTCCCAACTGTCTCTCCA | 64 | 312 |
R - ACCTGGGCCTGTGTGTCC | |||
Exon 18-19 | F - TCTGAGAGGGAAGGGTCTGG | 64 | 443 |
R - CCCTTCTCACTTCCCCCTCA | |||
MATN3 gene | |||
Exon 3 | F - AAAGGAGCCCAGAGAGCAAT | 59 | 290 |
R - CAGTCCAAAACCTGGAGCAT | |||