Skip to main content

Table 2 Primer sequences used for real-time PCR

From: Increased CCL19 and CCL21 levels promote fibroblast ossification in ankylosing spondylitis hip ligament tissue

 

Sense primer (5′ to 3′)

Anti-sense primer (5′ to 3′)

Human CCL19

CCAACTCTGAGTGGCACCAA

TGAACACTACAGCAGGCACC

Human CCL21

TGGCCTCTTACTCACCCTCT

GCCTCTTGATCCCCTTAGCC

Human CCR7

GCCTACGACGTCACCTACAG

GGCAGAAGAGTCGCCTATGG

Beta-actin

CCATCGTCCACCGCAAAT

TGTCACCTTCACCGTTCC

ALP

ATCTTTGGTCTGGCTCCCATG

TTTCCCGTTCACCGTCCAC

Human OCN

GGCAGCGAGGTAGTGAAGAG

CTGGAGAGGAGCAGAACTGG

Human COL1

GAG AGC ATG ACC GAT GG

GTG ACG CTG TAG GTG AA

IBSP

CGCCAATGAATACGACAATG

GATGCAAAGCCAGAATGGAT

Human VEGF-A

GAGCCTTGCCTTGCTGCTCTA

CACCAGGGTCTCGATTGGAT

Runx-2

GCAGCAACCCAGAAACACTT

AACACATGACCCAGTGCAAA

Osterix

AGAGGAGAGACTCGGGACAG

GAGTTGTTGAGTCCCGCAGA

  1. CCL19: Chemokine (C-C motif) ligand 19; CCR7: Chemokine receptor 7; ALP: Alkaline phosphatase; OCN: Osteocalcin; COL1: Collagenase I; IBSP: Integrin-binding sialoprotein; VEGF-A: Vascular endothelial growth factor-A; Runx-2: Runt-related transcription factor-2.
Back to article page

BMC Musculoskeletal Disorders

ISSN: 1471-2474

Contact us