Figure 5

Immunostaining of C-Col10 and C2M in human OA knee cartilage sections. C- Col10 was observed in the deep zone around the pre- hypertrophic chondrocytes in mild OA, and around chondrocyte clusters in severe OA, whereas C2M was observed in all layers and consistently in 3 OA cartilage sections. A, B, C: Safranin O/ Fast green histology staining, 4× magnification, scale bar 500 μm; D, E, F: stained with NB509-11G8 antibody, 4× magnification, scale bar 500 μm; G, H, I: stained with NB509-11G8 antibody, 10x magnification, scale bar 200 μm. J, K, L: stained with C2M antibody, scale bar 500 μm; M, N, O: stained with C2M antibody, 10× magnification, scale bar 200 μm. P, Q, R: normal mouse IgG, negative control, scale bar 500 μm.