Matrix forming characteristics of inner and outer human meniscus cells on 3D collagen scaffolds under normal and low oxygen tensions

Table 1 Primer sequences used for real-time PCR analysis

Gene	Primer Sequence	Direction	Reference
Aggrecan	5′AGGGCGAGTGGAATGATGTT3′	Forward	32
Aggrecan	5′GGTGGCTGTGCCCTTTTTAC3′	Reverse	32
COL1A2	5′TTGCCCAAAGTTGTCCTCTTCT3′	Forward	32
COL1A2	5′AGCTTCTGTGGAACCATGGAA3′	Reverse	32
COL2A1	5′CTGCAAAATAAAATCTCGGTGTTCT3′	Forward	32
COL2A1	5′GGGCATTTGACTCACACCAGT3′	Reverse	32
COMP	5′GCGAAACGTGGGTTGGAA3′	Forward	36
COMP	5′GCCGGTGCTGCAGGAA3′	Reverse	36
HIF-1α	5′GTAGTTGTGGAAGTTTATGCTAATATTGTGT3′	Forward	34
HIF-1α	5′CTTGTTTACAGTCTGCTCAAAATATCTT3′	Reverse	34
HIF-2α	5′GGTGGCAGAACTTGAAGGGTTA3′	Forward	35
HIF-2α	5′GGGCAACACACACAGGAAATC3′	Reverse	35
Sox9	5′CTTTGGTTTGTGTTCGTGTTTTG3′	Forward	32
Sox9	5′AGAGAAAGAAAAAGGGAAAGGTAAGTTT3′	Reverse	32
β-actin	5′AAGCCACCCCACTTCTCTCTAA3′	Forward	32
	5′AATGCTATCACCTCCCCTGTGT3′	Reverse

All primers were purchased from Invitrogen (Mississauga, Ontario, Canada). COL1A2, collagen type I α2 chain; COL2A1, collagen type II α1 chain; COMP, cartilage oligomeric matrix protein; HIF-1α, hypoxia-inducible factor 1α; HIF-2α, hypoxia-inducible factor 2α; Sox9, Sry-related HMG box 9.

ISSN: 1471-2474