Figure 3From: Matrix forming characteristics of inner and outer human meniscus cells on 3D collagen scaffolds under normal and low oxygen tensions Expression of fibrocartilaginous markers at P3 versus D21. Inner and outer MFCs were expanded to Passage 3 (P3, white bars) then chondrogenically stimulated in scaffold culture under 21% or 3% O2 in vitro for 21 days (D21, hatched bars). mRNA transcript expression levels were analyzed for collagen type I (Col1), collagen type II (Col2), aggrecan and cartilage oligomeric matrix protein (COMP). Expression was normalized to levels of the housekeeping gene β-actin using 2-ΔCT method. * = p < 0.05 ** = p < 0.01 represents significant difference between P3 and D21 values within groups using Student’s two-tailed t-test. There was no significant difference in expression levels of these mRNA transcripts between D21 scaffold constructs from inner or outer MFCs cultured under 21% or 3% oxygen tension. # = p <0.05 by one-way ANOVA with Games-Howell multiple comparisons post-test.Back to article page