Figure 9

Depth-wise measures of osteoclast chemotaxis and quantification of non-mineralized bone hole dimensions. (A) Total number of TRAP+ cells lining both sides of each hole were quantified in three segments of each hole: the bone plate (tidemark-0.5mm deep), mid-level (0.5-1.0 mm deep) and deeper level (1.0-1.5mm deep). Compared to controls, treated defects showed more osteoclasts and osteoclast precursors in the bone plate region 21 days post-treatment (N=4). (B) Hole cross-sectional area at four depths (tidemark, 0.5 mm depth, 1.0 mm depth and 1.5 mm depth) in 3D microCT reconstructed scans in defects from 1 day (N=1) or 21 days (N=4) post-treatment. Treated drill holes became larger, and bone remodeling was particularly strong in 150K-treated defects at 0.5 mm and 1.5 mm depths compared to 40K-treated after 21 days. (C) Depth of the drill holes in micro-CT scans showed that middle control holes, after 21 days (N=4), were significantly more repaired at the base than the matching 40K-treated defects. (mean ± standard deviation). *p<0.05, **, ††p<0.01, ***, ###p<0.001.