Table 1 Primer sequences of target genes for real-time PCR
From: Aging is associated with increased activities of matrix metalloproteinase-2 and -9 in tenocytes
Target genes | Primer sequence |
|---|---|
GAPDH | 5’ AGTCTACTGGCGTCTTCA 3’ forward |
|  | 5’ TTGTCATATTTCTCGTGGT 3’ reverse |
COL1A1 | 5’ TACAGCACGCTTGTGGATG 3’ forward |
|  | 5’ TTGGGATGGAGGGAGTTTA 3’ reverse |
MMP-2 | 5’ GGAAGCATCAAATCGGACTG 3’ forward |
|  | 5’ GGGCGGGAGAAAGTAGCA 3’ reverse |
MMP-9 | 5’ CCCACTTACTTTGGAAACG 3’ forward |
|  | 5’ GAAGATGAATGGAAATACGC 3’ reverse |
TIMP-1 | 5’ GCCTCTGGCATCCTCTTG 3’ forward |
|  | 5’ CTGCGGTTCTGGGACTTG 3’ reverse |
TIMP-2 | 5’ CCAAAGCAGTGAGCGAGAA 3’ forward |
|  | 5’ CCCAG GGCAC AATAA AGTC 3’ reverse |
TGF-beta-1 | 5’ AGAGATTCAAGTCAAACTGTGGAG 3’ forward |
|  | 5’ CCAAGGTAACGCCAGGAA 3’ reverse |