Figure 10

Double-staining for tachykinin/white blood cell markers. Sections of soleus muscle tissue of the 6w group, non-exercised side (A-C) and section of gastrocnemius muscle tissue of the 1w group, exercised side (D,E). Double stainings for tachykinin (sc-14104) (green) and neutrophil/T-cell marker (MCA805G) (yellowish) are shown in (A) and (D,E). In (B), double staining for tachykinin (green) and macrophage marker (CD68) (yellowish) is shown and in (C), double staining for tachykinin and eosinophil marker (yellowish) is demonstrated. A white blood cell shows immunoreactivity for the neutrophil/T-cell marker (arrow) but does not show tachykinin-like immunoreactivity (A). There is on the other hand tachykinin-like immunoreactivity (green) in the smooth muscle of a vessel wall (asterisk); the sign (#) depicts debris material within the lumen of the blood vessel (A). There are unspecific reactions in the endothelium of the vessel wall (A). Note that tachykinin-like immunoreactivity (green) can be seen in the macrophage (arrowhead) in (B). There is a blood vessel, in which wall there is only tachykinin-like immunoreactivity (asterisk) (B). There is also tachykinin-like immunoreactivity in eosinophils (arrowheads) (C). Concerning (D,E): The occurrence of reactions for white blood cells after processing for MCA805G is shown in (D) (arrows). The combination of reactions for tachykinin/DAPI is shown in (E). The same cells as indicated in (D) are indicated in (E) (arrows). Note that the cells are not exhibiting tachykinin-like immunoreactivity. There is on the other hand tachykinin-like immunoreactivity in the vessel wall in (E) (asterisk). The reaction for MCA805G is masked in (E). Magnification x315.