Table 1 Antibodies used for the immunocytochemical and immunofluorescence analyses
PRODUCT NAME | PRODUCT CODE | ANTIGEN | DILUTION IMMUNOCYTO CHEMISTRY/IMMUNOFLUORESCENCE | DILUTION SECONDARY ANTIBODY | COMPANY |
|---|---|---|---|---|---|
Mouse monoclonal to desmin | M0760 | Desmin | 1:100 | 1:1000 Anti-mouse Dylight 549 | DakoCytomation |
Mouse monoclonal [F5D] to myogenin | Ab1835 | Myogenin | 1:50 | Â | Abcam plc |
Mouse monoclonal to troponin T, fast | NCL-TROPT | TNNT | 1:20 | Â | NovoCastraâ„¢Lyophilized |
Mouse monoclonal to titin | 3010-S | Titin | 1:50 | Â | BioCytex |
Mouse monoclonal to sarcomeric actin | M0874 | Alpha-skeletal actin | 1:10 | 1:1000 Anti-mouse Dylight 549 | DakoCytomation |
Mouse monoclonal cardiac actin | M622709 | Alpha-cardiac actin | 1:20 | 1:1000 Anti-mouse Dylight 549 | Nordic Biolabs Bioreagents |
Mouse monoclonal to myosin heavy chain, (developmental) | NCL-MHCd | MyHC-embryonic | 1:10 | 1:1000 Anti-mouse Dylight 549 | NovoCastraâ„¢Lyophilized |
Mouse monoclonal to myosin heavy chain, (neonatal) | NCL-MHCn | MyHC-neonatal | 1:10 | 1:1000 Anti-mouse Dylight 549 | NovoCastraâ„¢Lyophilized |
Mouse monoclonal to myosin heavy chain, (slow) | NCL-MHCs | MyHC-slow | 1:250 | Â | NovoCastraâ„¢Lyophilized |
Mouse monoclonal to myosin heavy chain, (fast) | NCL-MHCf | MyHC-fast | 1:120 | Â | NovoCastraâ„¢Lyophilized |
Mouse monoclonal to myosin heavy chain, (fast IIa and slow) | N2.261 | MyHC-fast IIa+slow | 1:120 | 1:1000 Anti-mouse Dylight 549 | Santa Cruz Biotechnology |
Mouse monoclonal to sarcomeric tropomyosin | T9283 | TPM | 1:100 | Â | Sigma-Aldrich |
Rabbit polyclonal to beta tropomyosin | ARP48224T100 | TPM2 | 1:120 | Â | Aviva Systems Biology |
Rabbit polyclonal to myosin binding protein C, (slow) | HPA021004 | MYBPC1-slow | 1:50 | Â | Sigma-Aldrich |
Rabbit polyclonal to myosin binding protein C, (fast) | SAB2101539 | MYBPC2-fast | 1:100 | Â | Sigma-Aldrich |
Mouse monoclonal [12F10] to skeletal muscle troponin I, (slow) | Ab8293 | TNNI-slow | 1:500 | Â | Abcam plc |
Mouse monoclonal [2F12A11] to skeletal muscle troponin I, (fast) | Ab119943 | TNNI- fast | 1:200 | Â | Abcam plc |
Mouse monoclonal [284(19C7)] to cardiac troponin I | Ab19615 | TNNI- cardiac | 1:500 | Â | Abcam plc |
Mouse monoclonal T12 to Z-disk titin |  | Z-disk | 1:20 | Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 | Fürst et al., 1988 |
Mouse monoclonal T3 to A/I junction titin |  | A/I junction | 1:5 | Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 | Fürst et al., 1988 |
Mouse monoclonal T30 to A-band titin |  | A-band | 1:5 | Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 | Fürst et al., 1989 |
Mouse monoclonal T51 to M-band titin | Â | M-band | 1:5 | Polyclonal Anti-Mouse Immunoglobulins/FITC 1:1000 | Obermann et al., 1996 |