Figure 3

Typical photomicrographs showing the localizations of myoD and pax7 at day 2 and their quantifications. Triple immunostaining (A) of myoD (green), laminin (red), nuclei (blue), and merge for myoD-negative/pax7-positive, myoD-positive/pax7-negative, and myoD-positive/pax7-positive. Arrows indicate immunoreactive positive regions. Bar = 30 μm. The graph (B) shows the percentages of myoD-negative/pax7-positive cells (red bar), myoD-positive/pax7-negative (blue bar), and myoD-positive/pax7-positive (black bar). ***: p < 0.001, compared to pre-treatment and day 7 levels of the percentages of myoD-positive/pax7-positive cells.