Table 1 Primer sequences for Col II, Col X, aggrecan, ERα, ERβ, aromatase and S18
Gene | Sequence | Fragment length (bp) | Accession number |
|---|---|---|---|
Col II | F: 5’-AGAACTGGTGGAGCAGCAAGA-3' | 124 bp | NM_012929 |
R: 5’-ATCTGGACGTTAGCGGTGTTG-3’ | |||
Col X | F: 5’- CCATGGTTCACACAACCCCTT -3’ | 129 bp | AJ131848.1 |
R: 5’- TGGCTGTGGTAAAGCACCTTG -3’ | |||
Aggrecan | F: 5’-CCCTCACCCCAAGAATCAAGT-3’ | 178 bp | NM_022190 |
R: 5’- TCATTGGAGCGAAGGTTCTGG-3’ | |||
ERα | F: 5’-TGCGCAAGTGTTACGAAGTGG-3’ | 108 bp | NM_012689 |
R: 5’-TTCGGCCTTCCAAGTCATCTC-3’ | |||
ERβ | F: 5’-AAAAACTCACCGTCGAGCCTT-3’ | 124 bp | NM_012754 |
R: 5’-GCTGAATACTCATGGCGGTTG-3’ | |||
Aromatase | F: 5’-TCATCAGCAAGTCCTCGAGCA-3’ | 106 bp | M33986 |
R: 5’-CCATTCTCGTGCATGCCAAT-3’ | |||
S18 | F: 5’-CGGCTACCACATCCAAGGAA-3’ | 187 bp | M11188 |
R: 5’-GCTGGAATTACCGCGGCT-3’ |