Table 1 RT-PCR primer sequences and product length
From: Collagen type I and decorin expression in tenocytes depend on the cell isolation method
Target gene | Primer sequence | Length |
|---|---|---|
1. GAPDH [NM_ 002046] | Sense: GAGTCCACTGGCGTCTCCAC | 188 bp |
Antisense: GGTGCTAAGCAGTTGGTGGT | Â | |
2. collagen Typ I, alpha 1 [NM_000088] | Sense: GGCCCAGAAGAACTGGTACA | 200 bp |
Antisense: GGCTGTTCTTGCAGTGGTAG | Â | |
3. collagen Typ III, alpha 1 [NM_033150] | Sense: CCAGGAGCTAACGGTCTCAG | 103 bp |
Antisense. CAGGGTTTCCATCTCTTCCA | Â | |
4. decorin [NM_001920] | Sense: TGCTGTTGACAATGGCTCTC | 192 bp |
Antisense: GCCTTTTTGGTGTTGTGTCC | Â | |
5. fibronectin [NM_212475] | Sense: ATGATGAGGTGCACGTGTGT | 135 bp |
Antisense: CTCTTCATGACGCTTGTGGA | Â | |
6. tenascin-C [NM_002160] | Sense: TCAAGGCTGCTACGCCTTAT | 230 bp |
Antisense: GTTCTGGGCTGCCTCTACTG | Â | |
7. Scleraxis[17] | Sense: CCTGAACATCTGGGAAATTTTAC | 111 bp |
Antisense: CGCCAAGGCACCTCCTT | Â | |
8. tenomodulin [NM_022144] | Sense: CCATGCTGGATGAGAGAGGT | 123 bp |
Antisense: CTCGTCCTCCTTGGTAGCAG | Â | |
9. osteopontin[26] | Sense: TTGCTTTTGCCTCCTAGGCA | 430 bp |
Antisense: GTGAAAACTTCGGTTGCTGG | Â | |
10. aggrecan[27] | Sense: CACTGTTACCGCCACTTCCC | 183 bp |
Antisense: ACCAGCGGAAGTCCCCTTCG | Â |