Table 1 Primer Used in Real Time RT-PCR Analysis
From: Proteasome inhibition alleviates prolonged moderate compression-induced muscle pathology
Gene | Accession Number | Primers |
|---|---|---|
| Â | Â | (F; forward primer, R; reverse primer) |
Ubiquitin | NM017314 | F: 5'GGGCATGCAGATCTTTGTGAA3' |
| Â | Â | R: 5'ACCTCCAGGGTGATGGTCTTG3' |
MAFbx/atrogin-1 | NM133521 | F: 5'TGAAGACCGGCTACTGTGGAAGAGAC3' |
| Â | Â | R: 5'TTGGGGTGAAAGTGAGACGGAGCAG3' |
HIF-1α | NM024359 | F: 5'AACAAACAGAATCTGTCCTCAAACC3' |
| Â | Â | R: 5'CAGGTAATGGAGACATTGCCAG3' |
HSP70-1 | X77207 | F: 5'CGAGGGCATCGACTTCTACACG3' |
| Â | Â | R: 5'ATCTGCGCCTTGTCCAGCTTG3' |
HSP70-2 | X77208 | F: 5'CTCGTCCATGGTGCTGACCAAG3' |
| Â | Â | R: 5'CCGCTGCGAGTCGTTGAAGTAG3' |
HSP70-3 | X77209 | F: 5'AGGACTCAACGTGCTGCGAATC3' |
| Â | Â | R: 5'TCAGGATGGACACGTCGAACG3' |
β2M | NM012512 | F: 5'CGTGATCTTTCTGGTGCTTGTC3' |
| Â | Â | R: 5'TTCTGAATGGCAAGCACGAC3' |