Molecular changes in articular cartilage and subchondral bone in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis

Pickarski, Maureen; Hayami, Tadashi; Zhuo, Ya; Duong, Le T

doi:10.1186/1471-2474-12-197

Table 1 Oligonucleotides and TaqMan fluorogenic probes

From: Molecular changes in articular cartilage and subchondral bone in the rat anterior cruciate ligament transection and meniscectomized models of osteoarthritis

Gene	Genebank	Primer	Sequence 5'-3'	Amplicon
Aggrecanase-1	NM_023959	Forward	CTGCATCTGCCAGTGACTTTTC	74
		Reverse	TCAGGACCAAAGGTCAGTTGG
		Probe	TGGCAAGGACTATGATGCTGACCGC
Bone Sialoprotein	NM_012587	Forward	GTTGGAGTTAGCTGCGCTCC	90
		Reverse	TCCTCTTCCTCGTCGCTTTCCTTCATT
		Probe	AAGGCTGGAGATGCAGAGGGCAAGG
Cathepsin K	NM_031560	Forward	GCCATGAATCACCTGGGAGA	76
		Reverse	GCGAAGGTGGCACTCTGAGT
		Probe	TGA CCAGCGAAGAAGTGGTTCAGAAGATGA
Cbfa-1/Runx2	AF325502	Forward	TGCTTCATTCGCCTCACAA	71
		Reverse	CTTGCTGTCCTCCTGGAGAAA
		Probe	AACCACAGAACCACAAGTGCGGTGC
CD31	NM_031591	Forward	TCAACAGAGCCAGCATTGTGA	70
		Reverse	CACGGAGCAAGAAAGACTCTGA
		Probe	CAGTCTCCGAAGCGGCCCTCTAACA
Collagen Type IIA	NM_031163	Forward	TCTGCAGAATGGGCAGAGGTATA	103
		Reverse	GATAATGTCATCGCAGAGGACATTC
		Probe	AAGCCCTCATCTTGCCGCATCTGTG
Cyclophilin A	NM_017101	Forward	CAAATGCTGGACCAAACACAA	70
		Reverse	GCCATCCAGCCACTCAGTCT
		Probe	TGGTTCCCAGTTTTTTATCTGCACTGCC
KDR	NM_013062	Forward	ACGTTTGAGAACCTCACGTGG	84
		Reverse	CTTGCAAACTGGTGTGAGTGATTC
		Probe	AGCTTGGCTCACAGGCAACATCGG
MMP-13	NM_133530	Forward	AGTCCTTTTGGCCAGAACTTCC	76
		Reverse	AAGATGAACATGAGGTCTCGGG
		Probe	CCATGTGGATGCTGCATACGAGCATC
Osterix	NM_181374	Forward	TGACTGCCTGCCTAGTGTCTACA	94
		Reverse	ACCTGGTGAGATGCCTGCA
		Probe	ATGTCCCATCCCTACGGCTCCTGGTA
Osteopontin	NM_012881	Forward	TCTGATGAACAGTATCCCGATGC	84
		Reverse	GACCTTGATAGCCTCATCGGAC
		Probe	AGGACCTCACCTCCCGCATGAAGAG
Sox-9	AB073720	Forward	CGCAGGAAGCTGGCAGAC	76
		Reverse	GTCTCCAGAGCTTGCCCAGA
		Probe	CCGCATCTGCACAACGCGGA
TRAP	NM_019144	Forward	AATTGCCTACTCCAAGATCTCCAA	74
		Reverse	GCGGAACTTTGAAACGCAAA
		Probe	CGCTGG AACTTCCCCAGCCCTTATTA
VEGF	NM_031836	Forward	AGCCCATGAAGT GGTGAAGTTC	71
		Reverse	CCACCAGGGTCTCAATTGGA
		Probe	TGGACGTCTACCAGCGCAGCTATTGC

^{Exon/intron boundaries are indicated with underlined bases. Exon/intron boundaries for Cathepsin K, TRAP, MMP-9, and VEGF are assumed by comparison to mouse sequence.}

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ISSN: 1471-2474

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