Figure 7
From: Tenascin-C induces inflammatory mediators and matrix degradation in osteoarthritic cartilage
TN-C in human synovial fluids and its correlation to proteoglycan release. A, Western blot analysis of human synovial fluid. Representative samples (15 μl each) were treated with 10 U of hyaluronidase overnight at RT, separated on a 3-8% tris-acetate gel, transferred, and probed with anti-TN-C (EGF-like domain) antibody, 4F10TT that detects all forms of TN-C. Lanes are N = synovial fluid of non-OA reference subjects; O = synovial fluid of OA patients. Bands are marked as L for large variants and S for small variants. B, Correlation analysis of TN-C vs. ARG-aggrecan in human synovial fluid. C, Correlation analysis of TN-C vs. total proteoglycan in human synovial fluid. For both B and C, N = 8 each group; triangles represent non-OA reference subjects and circles represent OA patients.