Figure 4

Pharmacologic inhibition of PI3Kγ alleviates joint swelling and macrophage activity. Wildtype C57BL/6 mice were immunized with mBSA, AIA was induced and animals randomly assinged to two groups, receiving either AS-605240 or vehicle (saline) for 7 consecutive days. (A) Knee swelling was measured as a clinical marker of AIA. n = 5, *: p < 0.05, ** p < 0.01 (B) Peritoneal macrophages were isolated at day 2 of AIA, cultured for 24 hours without restimulation and production of effector molecules was measured in the culture supernatant (mean and SEM of triplicates). (C) Splenocytes and lymph node cells were isolated at day 2 and 7 of AIA, cultured for 24 hours in the presence of mBSA and cytokines were measured in the supernatants.