Figure 3
From: Investigation of the direct effects of salmon calcitonin on human osteoarthritic chondrocytes
Human chondrocytes express the calcitonin receptor. A. Calcitonin receptor expression in human osteoclasts and cartilage was analyzed using QPCR. QPCR products were visualized on a 2% agarose gel. Lanes: 1+19 NEB 100 bp DNA ladder, 2-4 CalcR1, 5-7 CalcR2, 8-10 CalcR 3, 11-13 CalcR 4, 14-16 Surf 1, 17-18 Coll2a. Abbreviations: CalcR - calcitonin receptor primer pair 1-4; Coll2a - Collagen type II; HOC - human osteoclasts; HCC - human chondrocytes; NTC - no template control. B. Intracellular cAMP levels after stimulation with salmon calcitonin: Human OA chondrocytes were stimulated with IBMX for one hour, followed by 15 minutes stimulation in presence or absence of salmon calcitonin as specified in the figure legends. C. Binding competition by the calcitonin receptor antagonist 8-32sCT. Forskolin was used as positive control. The asterisks indicate significant differences (** P < 0.01, *** P < 0.001).