Coadministration of minocycline with indomethacin enhances their effects and alleviates LPS-induced hyperalgesia. Effect of treatment with indomethacin, minocycline or a combination of minocycline plus indomethacin on LPS-induced hyperalgesia at day 7 post lipopolysaccharide (LPS, 1 mg/kg, i.p.) inoculation in a hot plate test in BALB/c mice. A: Hypernociceptive effects of LPS, 1 mg/kg, i.p. in the hot plate model of inflammatory thermal hyperalgesia in BALB/c mice at 7 days post inoculation. Each point represents the mean ± S.E.M of the values obtained from 6 vehicle-treated and 29 LPS-treated animals. Statistically significant differences in comparison with vehicle inoculated mice at the same time point post treatment: ** p < 0.01 (two-way ANOVA followed by Bonferroni test). B: Percentage change in reaction latency times from baseline values (taken at 7 days post LPS) at 1 hour after treatment with minocycline (50 mg/kg, n = 15), indomethacin (10 mg/kg, n = 14), minocycline + indomethacin (n = 16) or their vehicles (n = 12) in hot plate test. Each bar represents the mean ± S.E.M of the values obtained from 12 to 16 animals. Statistically significant differences in comparison with drug vehicle: * p < 0.05 (two-way ANOVA followed by Bonferroni test).