Table 1 Synthetic gene-specific primer sequences for collagen types I and III and for TGF-β1, -β2, -β3 and β-actin
Gene | Primers | Product Size | |
|---|---|---|---|
Collagen types I | Forward | GTCGAGGGCCAAGACGAAG | 143 bp |
Reverse | CAGATCACGTCATCGCACAAC | ||
Collagen type III | Forward | TGGTCCCCAAGGTGTCAAAG | 117 bp |
Reverse | GGGGGTCCTGGGTTACCATTA | ||
TGF-β1 | Forward | GGCCAGATCCTGTCCAAGC | 201 bp |
Reverse | GTGGGTTTCCACCATTAGCAC | ||
TGF-β2 | Forward | TGCCATCCCGCCCACTTTC | 252 bp |
Reverse | GAGAGCCATTCGCCTTCTGC | ||
TGF-β3 | Forward | CTGTGCGTGAGTGGCTGTTG | 406 bp |
Reverse | GTGCTGTGGGTTGTGTCTGC | ||
β-actin | Forward | ATCGTGCGTGACATTAAGGAGAAG | 179 bp |
Reverse | AGGAAGGAAGGCTGGAAGAGTG |