Figure 6

Negative coupling to hyaluronan secretion in primary cultures of FLSs from patients with RA. Data are from measurements of hyaluronan derived from cells of 6 (a-c) or 3 (d) patient samples. (a) Mean effects of 100 μM pregnenolone sulphate (PregS), 10 ng/ml tumor necrosis factor (TNF) and 1 μg/ml dexamethasone; 'control' was the solvent. (b) Mean data showing no effect of anti-TRPM3 antibody (TM3E3) on basal hyaluronan secretion; one control was without TM3E3 (no Ab) and another was from cells pretreated with TM3E3 preadsorbed to its antigenic peptide (TM3E3 + pep.). (c) As for (b) but showing the effect 100 μM PregS under the different antibody (Ab) conditions; the control was cells without PregS. (d) Failure of TM3E3 to protect against the inhibitory effect of 1 μg/ml dexamethasone.