Figure 2

Sphingosine-evoked Ca ²⁺ signals in HIG-82 cells. Data were generated by intracellular Ca²⁺ measurement. Responses elicited by (a) sphingosine (SPH, 20 μM), (b) dimethylsphingosine (DMS, 20 μM) or (c) adenosine triphosphate (ATP, 100 μM) were tested in either 1.5 mM Ca²⁺-containing (+Ca²⁺) or Ca²⁺-free extracellular solution. Responses are each representative of 3 (n) independent experiments.